The disinfection of surfaces containing biofilms is complex, since bacteria within biofilms use mechanisms of protection that lead to an increased bacterial resistance against commonly used disinfectants. To eliminate biofilm contamination, microorganisms within the biofilm must be destroyed and the biofilm structure itself must be removed from the surface. The study presented here uses a standard method (ASTM standard test method E2871-12) for measuring efficacy of six disinfectants (Hydrogen peroxide, peracetic acid, chlorine dioxide, sodium hypochlorite, quaternary ammonium compound and enzyme) on removing biofilm and killing the bacteria within it. Results show that some disinfectants are good to kill bacteria (e.g., sodium hypochlorite) where other are better at removing the biofilm structure from the surface (e.g., enzyme). However, two disinfectants, peracetic acid and chlorine dioxide, are able to have a dual action on killing the bacteria and removing the biofilm from the surface. We also conclude that diffusion controls the relative efficacy of each disinfectant.